Thermo Scientific SuperSignal ELISA Femto Maximum Sensitivity Substrate is formulated for superior protein detection and low-end linearity in chemiluminescent ELISA applications.

SuperSignal ELISA Substrate uses an improved enhancer system that meets the needs of high throughput screening and diagnostic assays. Researchers looking for greater sensitivity in their ELISAs or any other solution-based assay are now able to use chemiluminescent substrates for enzyme detection and quantification. These ELISAs can take place in either a test tube or a microplate and are quantified by measuring relative light units (RLU) in a luminometer.

Highlights:

• Immediate light generation – intense signal generated immediately at both room temperature and 37°C; emits light at 425nm
• Improved low-end linearity – easy detection of low quantities of proteins with high signal:noise ratios and low-end linearity of dose response curves
• High sensitivity – femtogram-level detection of target proteins in an ELISA
• Reduction in assay time – high sensitivity allows for reduction in ELISA incubation steps
• Stability – storage for six months at room temperature or a minimum of 12 months at 4°C with a six-hour working solution stability