SIRT3 Rabbit Polyclonal Antibody (KO Validated)-抗体-抗体-生物在线
SIRT3 Rabbit Polyclonal Antibody (KO Validated)

SIRT3 Rabbit Polyclonal Antibody (KO Validated)

商家询价

产品名称: SIRT3 Rabbit Polyclonal Antibody (KO Validated)

英文名称: SIRT3 Rabbit Polyclonal Antibody (KO Validated)

产品编号: K5303

产品价格: null

产品产地: 上海

品牌商标: 雅吉生物

更新时间: null

使用范围: WB, IF, ICC

上海雅吉生物科技有限公司
  • 联系人 :
  • 地址 : 上海市闵行区元江路5500号第1幢5658室
  • 邮编 :
  • 所在区域 : 上海
  • 电话 : 158****3937
  • 传真 : 021-34661275
  • 邮箱 : yajikit@163.com

来源用途交叉反应性理论分子量实际分子量
RabbitWB, IHCH, M, R28/43kDa28kDa

WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay; ChIP-seq, ChIP-sequencing.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀释液,可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整):

WBIPIFIHCICCFCChIPChIP-seq
1:500-1:1000--1:20-1:501:20-1:50---

抗体详细信息如下::

About this Antibody
NameSIRT3 Rabbit Polyclonal Antibody (KO Validated)
CategoryRabbit Polyclonal Antibody (pAb); Primary antibody
IsotypeIgG
Purification methodAffinity purification
Positive samplesSW620
Cellular locationMitochondrion matrix
Customer validationWB (Human)
About the Immunogen
ImmunogenA synthetic synthetic peptide of human SIRT3 (NP_036371.1).
SequenceQRFLLHVVDFPMADLLLILGTSLEVEPFASLTEAVRSSVPRLLINRDLVGPLAWHPRSRDVAQLGDVVHGVESLVELLGWTEEMRDLVQRETGKLDGPDK
Gene ID23410
Swiss ProtQ9NTG7
SynonymsSIRT3; SIR2L3; sirtuin 3
CategoryProtein Acetylation; Fatty acid pathway; Epigenetics
BackgroundThe Silent Information Regulator (SIR2) family of genes is a highly conserved group of genes that encode nicotinamide adenine dinucleotide (NAD)-dependent protein deacetylases, also known as Class III histone deacetylases. The first discovered and best characterized of these genes is Saccharomyces cerevisiae Sir2, which is involved in silencing of mating type loci, telomere maintenance, DNA damage response and cell aging. SIRT3, a mammalian homolog of Sir2, exists in human cells in two forms. The full-length 44 kDa protein localizes to the nucleus, while a processed 28 kDa protein lacking 142 amino terminal residues localizes exclusively to the mitochondria. The single murine form of SIRT3 is equivalent to the processed human SIRT3 protein. Full-length SIRT3 protein is processed in the mitochondrial matrix by the mitochondrial matrix processing peptidase (MMP). Both full-length and processed forms of SIRT3 are enzymatically active and de-acetylate histone H3 at Lys9 and histone H4 at Lys16 in vitro . SIRT3 also de-acetylates Lys642 of acetyl-CoA synthetase 2 (AceCS2) and activates AceCS2 activity in the mitochondria. Restricted caloric intake, which is linked to increased lifespan in multiple organisms, increases SIRT3 expression in white and brown adipocytes of obese mice, suggesting a role for SIRT3 in aging. Two observations implicate SIRT3 in the regulation of mitochondrial thermogenesis. First, exposure to cold temperatures increases SIRT3 expression in brown adipocytes, while elevated temperatures reduce SIRT3 expression. Second, over-expression of SIRT3 results in increased levels of the mitochondrial uncoupling protein 1 (UCP1). SIRT3 protein levels are also elevated in certain breast cancers.

包装清单:

产品编号产品名称包装
AF5303SIRT3 Rabbit Polyclonal Antibody (KO Validated)50μl
AZ050Western一抗稀释液50ml
说明书1份

保存条件:
SIRT3 Rabbit Polyclonal Antibody (KO Validated) -20ºC保存,Western一抗稀释液-20ºC或4ºC保存,一年有效。Western一抗稀释液优先推荐4ºC保存,长期不使用可以考虑-20ºC保存,但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项:
如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验,请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体,包括已经使用过的稀释抗体,请4℃保存。
回收后重复使用的抗体,使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象,可以10,000g离心1-3分钟,取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况,则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果,请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。

代表性图片:

Fig. 1. Western blot analysis of extracts of SW620 cells, using SIRT3 antibody at 1:1000 dilution. Secondary antibody: HRP-labeled Goat Anti-Rabbit IgG(H+L) (A0208) at 1:1000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: QuickBlock™ Blocking Buffer (P0231). Detection: BeyoECL Star (P0018A). Exposure time:1s.Fig. 2. Western blot analysis of extracts from normal (control) and SIRT3 knockout (KO) HeLa cells, using SIRT3 antibody at 1:500 dilution. Secondary antibody: HRP-labeled Goat Anti-Rabbit IgG(H+L) (A0208) at 1:1000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: QuickBlock™ Blocking Buffer (P0231). Detection: BeyoECL Star (P0018A). Exposure time:1s.
Fig. 3. Immunohistochemistry of paraffin-embedded rat kidney using SIRT3 antibody at dilution of 1:100 (40x lens).Fig. 4. Immunohistochemistry of paraffin-embedded human liver cancer using SIRT3 antibody at dilution of 1:100 (40x lens).